Postgraduate Course: Practical Skills in Biochemistry B (PGBI11099)
Course Outline
| School | School of Biological Sciences |
College | College of Science and Engineering |
| Credit level (Normal year taken) | SCQF Level 11 (Postgraduate) |
Availability | Not available to visiting students |
| SCQF Credits | 10 |
ECTS Credits | 5 |
| Summary | The programme provides thorough training and hands-on experience in enhanced practical skills required for employment as a biochemical scientist. The course will cover a range of techniques from recombinant protein overexpression and purification, followed by activity assays and biophysical characterisation. |
| Course description |
Not entered
|
Entry Requirements (not applicable to Visiting Students)
| Pre-requisites |
|
Co-requisites | |
| Prohibited Combinations | |
Other requirements | None |
Course Delivery Information
|
| Academic year 2015/16, Not available to visiting students (SS1)
|
Quota: None |
| Course Start |
Semester 2 |
Timetable |
Timetable |
| Learning and Teaching activities (Further Info) |
Total Hours:
100
(
Lecture Hours 30,
Programme Level Learning and Teaching Hours 2,
Directed Learning and Independent Learning Hours
68 )
|
| Assessment (Further Info) |
Written Exam
0 %,
Coursework
100 %,
Practical Exam
0 %
|
| Additional Information (Assessment) |
Two written assessments:
Design experimental strategy fo biophysical and activity characterisation of purified protein (40%)
Experimental write-up, in the form, of a paper of practicals in 1-8 (60%)
|
| Feedback |
Not entered |
| No Exam Information |
Learning Outcomes
On completion of this course, the student will be able to:
- Extract and purify recombinant protein from E. coli by affinity chromatography
- Describe how and why differences in experimental conditions or available equipment will influence the experimental strategy and outcome
- Identify the applicable methods required and design an appropriate strategy for biophysical characterisation of a biomolecule
- Apply, or be able to explain the advantages and limitations of, a range of biophysical characterisation methods
- Design, implement and analyse a straightforward enzyme assay and describe the advantages and limitations of different methods for determining binding affinities
|
Additional Information
| Graduate Attributes and Skills |
Confidence in laboratory environment
Report writing
Data management
Appreciation of health and safety responsibilities
Time management
Organisation
Group work
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| Keywords | BioChemB |
Contacts
| Course organiser | Dr Janice Bramham
Tel: (0131 6)50 4704
Email: |
Course secretary | Ms Andrea Nichol
Tel: (0131 6)50 5538
Email: |
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