Postgraduate Course: Detailed Characterisation of Drug or Ligand Interactions Using Surface Plasmon Resonance (SPR) (BILG11005)
Course Outline
| School | School of Biological Sciences |
College | College of Science and Engineering |
| Credit level (Normal year taken) | SCQF Level 11 (Postgraduate) |
Availability | Not available to visiting students |
| SCQF Credits | 10 |
ECTS Credits | 5 |
| Summary | This course will provide both theoretical and some hands on-experience of the practical use of Surface Plasmon Resonance to characterise biophysical interactions, and will show how the affinity, kinetic and thermodynamic data generated from such experiments can be used to enrich and accelerate the progress of accurate SARs in the development of drugs and therapeutics. The data handling and processing of affinity/kinetic and thermodynamic data will be applicable to other biophysical characterisation techniques. |
| Course description |
Surface plasmon resonance (SPR) is routinely exploited in the kinetic analysis of biomolecular interactions and small-molecule drug-discovery/hit-validation studies. SPR instruments uniquely allow the measurement of kinetic and thermodynamic data specifically associated with complex formation and dissociation. Such SPR data can be used to "thermodynamic profile" interactions enhancing the correlation of solution binding measurements with structural features and the assignment of proportional energetic contributions to individual functional groups, the basis of the whole-structure-based design approach to engineered therapeutics and drug design. This course will provide both theoretical and some hands on-experience of the practical use of SPR to characterise biophysical interactions, and will show how the affinity, kinetic and thermodynamic data generated from such experiments can be used to enrich and accelerate the progress of accurate SARs in the development of drugs and therapeutics. The data handling and processing of affinity/kinetic and thermodynamic data will be applicable to other biophysical characterisation techniques.
Theoretical background of SPR.
Practical instrumental and experimental considerations for surface and data generation with small molecules and fragments.
Thermodynamic profiling
Data analysis - kinetic, affinity and thermodynamic.
Use of SPR data for small molecule:protein SARs.
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Entry Requirements (not applicable to Visiting Students)
| Pre-requisites |
|
Co-requisites | |
| Prohibited Combinations | |
Other requirements | None |
| Additional Costs | 0 |
Course Delivery Information
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| Academic year 2015/16, Not available to visiting students (SS1)
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Quota: None |
| Course Start |
Semester 2 |
Timetable |
Timetable |
| Learning and Teaching activities (Further Info) |
Total Hours:
100
(
Lecture Hours 30,
Programme Level Learning and Teaching Hours 2,
Directed Learning and Independent Learning Hours
68 )
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| Assessment (Further Info) |
Written Exam
0 %,
Coursework
100 %,
Practical Exam
0 %
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| Additional Information (Assessment) |
In Course Assessment - 100%
An ICA consisting of a data analysis of an example dataset of affinity, kinetic and thermodynamic data and generation of report describing development of further experiments/considerations for development of the theoretical drug/therapeutic based on the SARs generated/supplied. |
| Feedback |
Not entered |
| No Exam Information |
Learning Outcomes
| On successful completion of the course, students should be able to demonstrate an understanding of the technologies that underpin SPR instruments and experiments. They should also have demonstrated the ability to successfully generate and analyse an example dataset of affinity, kinetic and thermodynamic data. They should be able to discuss their results in a relation to the basic SARs of a model example protein:small molecule system.
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Additional Information
| Graduate Attributes and Skills |
Understanding of Surface plasmon resonance and its uses |
| Special Arrangements |
N/A |
| Study Abroad |
N/A |
| Keywords | SPR |
Contacts
| Course organiser | Dr Martin Wear
Tel:
Email: |
Course secretary | Miss Vicky Mactaggart
Tel: (0131 6)51 7052
Email: |
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